Molecular biology studies the life at the molecular level. In simpler words, molecular biology studies life as a manifestation of interactions of the macro and micro biomolecules.
The 2023 Noble Prize in Medicine or Physiology has been awarded to Katalin Kariko and Drew Wiesmann for their role in developing the first mRNA vaccines. The key discovery or their contribution was the in-vitro studies on mRNA and dendritic cells.
In-vitro produced mRNA is treated as a foreign body by the dendritic cells and releases certain signalling molecules that initiate the inflammatory responses. Their papers presented in 2008 and 2010 highlighted that the administration of mRNA with bases modified significantly boosted the synthesis of signalling molecules compared to unmodified mRNA.
Their discovery that the “base-modified mRNA was critical in deciding the inflammatory response of dendritic cells” paved roadways for the development of mRNA vaccines, which were critical in the development of vaccines in a shorter period of time to clutch the pandemic Covid-19.
The example mentioned above is related to the field of Molecular Biology, which signifies the importance of the field as an applied science.
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Molecular Biology is the study of molecular components of the cell, such as DNA, RNA, and proteins. Molecular biology deepens the understanding of gene expression, gene replication and their applications to contribute to the advancements in medicine, biotechnology, agriculture etc. The following are the topics that come under Molecular Biology.
The above-mentioned topics have significant importance in advancing medical sciences, biotechnological applications, evolutionary insights, cellular functions, advancing forensic tools, drug development and agricultural development.
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Types of RNA transcripts:
Transcription begins at the promoter region in a structural gene by an enzyme called RNA polymerase.
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Life at the molecular level is the interaction between macromolecules. The macromolecule that has the recipe of life encoded in them are the nucleotides. The
Nucleotides in all the organisms are made of DNA, with few exceptions like RNA viruses. The DNA in all organisms is double-stranded, with the exception of viruses with single-stranded DNA.
Organisms reproduce through gene transfer - at the molecular level, the event that plays an important role in gene transfer is DNA replication.
The living state of Living organisms is maintained by interactions of proteins with other macromolecules, including proteins. These proteins are made through two events, Transcription and Translation, that parallels the conversion of DNA into an mRNA, and then to proteins respectively.
Thus, life revolves around three important things, DNA replication, Transcription and Translation.
Transcription: The act of making a copy.
In genetics, this term refers to the process of synthesizing RNA from a DNA sequence.
The product of a gene is either a Protein or an RNA molecule.
The part of the gene that codes for a functional product is termed “Structural Gene”.
For a gene to be regulated and expressed, the structural gene must have certain regions that must define, the Beginning of a gene and the termination of the same gene.
The sequences that define the beginning of a gene are referred to as “Promoters”. They lie upstream to the Structural Gene.
The Sequences that define the termination of Transcription are “Terminators”. They lie downstream to the Structural Gene.
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